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Title: HPTLC identification of bioactive compounds and antioxidant activity of Pleurotus ostreatus and Lentinus edodes extracts
Authors: Bubueanu, Corina
Grigore, Alice
Serban, Ecaterina
Popa, Gabriela
Cornea, Petruta-Calina
Keywords: Mushrooms
Pleurotus ostreatus
Lentinus edodes
Issue Date: 2017
Publisher: University Politehnica of Bucharest
Abstract: Because of the special flavour and of the therapeutic properties, Pleurotus ostreatus and Lentinus edodes are among the most cultivated and consumed mushrooms species. This work aimed to evaluate the antioxidant properties and free radical scavenger activities of Pleurotus ostreatus and Lentinus edodes methanol extracts by phosphomolybdenum and DPPH- assays respectively, total phenolic content (Folin – Ciocalteu) and identification of bioactive compounds by HPTLC (high-performance thin layer chromatography). The obtained fingerprints of extracts, in tree systems (for phenols, coumarins and triterpenoid saponins) have shown the presence of ferulic acid as main compounds in Pleurotus ostreatus extract and ergosterol (provitamin D2) in both of them. Total phenol content was 2.03 g/100g for Lentinus edodes extract and 1.24 g/100g for Pleurotus ostreatus extract. Radical scavenger activity and total antioxidant capacity, was higher for Lentinus edodes extract (90.14%, 203.05 mg AA) in comparison with the results obtained for Pleurotus ostreatus extract (85.32%, 168.34mg AA). The mushrooms examined in this work could represent important and accessible sources of natural antioxidants for food, food supplement and cosmetic industry.
Description: Scientific Bulletin, Series F, Biotechnologies, vol. XXI, 2017, pp. 343-348
ISSN: 2285-1364
CD-ROM 2285-5521
on line 2285-1372
L 2285-1364
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