Please use this identifier to cite or link to this item:
http://hdl.handle.net/123456789/1716
Title: | Derivatization procedures and their analytical performances for HPLC determination in bioanalysis | Authors: | David, Victor Moldoveanu, Serban Galaon, Toma |
Affiliations: | University of Bucharest, Romania R. J. Reynolds Tobacco Co National Research and Development Institute for Industrial Ecology, ECOIND |
Keywords: | liquid chromatography; derivatization; bioanalysis; extractions; hydrophobicity; isotope-labeling | Issue Date: | Jan-2021 | Abstract: | Derivatization, or chemical structure modification, is often used in bioanalysis performed by liquid chromatography technique in order to enhance detectability or to improve the chromatographic performances for the target analytes. The derivatization process is discussed according to the analytical procedure used to achieve the reaction between the reagent and the target compounds (containing hydroxyl, thiol, amino, carbonyl, and carboxyl as the main functional groups involved in derivatization). Important procedures of derivatization used in bioanalysis are in situ, or procedures based on extraction processes (liquid-liquid, solid-phase and related techniques) applied to the biomatrix. In the review, the chiral, isotope-labeling, hydrophobicity-tailored, and post-column derivatizations are also included, based on the representative publications in the literature during the last two decades. Examples of derivatization reagents and brief reaction conditions are included, together with some bioanalytical applications and performances (chromatographic conditions, detection limit, stability, and sample biomatrix). |
Description: | Biomedical Chromatography, 2021, 35(1), SI, e5008 |
URI: | http://hdl.handle.net/123456789/1716 |
Appears in Collections: | Articles |
Files in This Item:
File | Description | Size | Format | Existing users please |
---|---|---|---|---|
review bmc e5008.pdf | 1.56 MB | Adobe PDF | Request a copy |
Google ScholarTM
Check
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.