The obtaining of high sensitivity and specificity transducer media for sensors specialized in biological toxin detection

Abstract

This paper presents the experimental results obtained as a result of the testing on castor of a new type of nanocomposite superparamagnetic material. The purpose of the biochemical fixing tests was to obtain information concerning the characteristics of the biochemical fixing: efficiency, mechanisms, etc. The tested nanocomposite structure represents a new nanocomposite material, of the (ECH)nβ-{Fe3O4-n[SiO1.5γ-(CH2)3(NH2)](NH2)nδ}-(GL)nε type [(ECH)-npa-(GL) → coated and polyvalently biochemically activated nanoparticles {n[SiO1.5γ-(CH2)3(NH2)](NH2)nδ = coating layer; (NH2)nδ,-NH2= shallow layer of amino groups (GL)nε= shallow molecular layer of glutaraldehyde (ECH)nβ= shallow molecular layer of epiclorhydrine}. In order to obtain eloquent information concerning the -NH2, =NH, -NH3OH, -SH, -OH, -S-S- haptene distribution, the magnetite nanoparticles were synthetised through the reversed mycelia method. Another main objective was to verify the concept of dynamic and in real time ’undestructive biochemical fixing’. The veridicity of this concept offers the possibility of applying npa-BC in thin sensitive layers, on interfaceable supports of the following types: thin oxyde and metallic layers, electronic transducers, interfaces amplifying the electric signal, etc.