Please use this identifier to cite or link to this item:
http://hdl.handle.net/123456789/793
DC Field | Value | Language |
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dc.contributor.author | Sora, Iulia | - |
dc.contributor.author | Galaon, Toma | - |
dc.contributor.author | David, Victor | - |
dc.contributor.author | Medvedovici, Andrei | - |
dc.date.accessioned | 2017-04-06T08:42:34Z | - |
dc.date.available | 2017-04-06T08:42:34Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 0035-3930 | - |
dc.identifier.uri | http://hdl.handle.net/123456789/793 | - |
dc.description | Revue Roumaine de Chimie Volume 52 Issue 5 | en_US |
dc.description.abstract | A selective, sensitive and accurate HPLC method for the assay of nimesulide and its major metabolite (4’-hydroxynimesulide) in human plasma samples is presented. The sample preparation procedure was based on a simple protein precipitation with acetonitrile. The reversed phase liquid chromatography was used as separation technique followed by UV detection of analytes at 300 nm. Separation was achieved on a Chromolith Performance RP 18e column using a mobile phase consisting of aqueous 0.2% triethylamine at pH 3.0 with H3PO4 and methanol (1:1, v/v). A small volume of plasma is required for the sample preparation procedure (200 μL), nitrazepam being used as internal standard. The limits of quantification for nimesulide and 4’-hydroxy-nimesulide are 64 ng/mL and 41 ng/mL, respectively. Relative standard deviations characterizing intra-day and inter-day precision were less than 5%. The validated method was applied during a bioavailability study for a pharmaceutical formulation containing nimesulide (uncoated tablet) available on the Romanian market. Both nimesulide and 4’-hydroxy-nimesulide were determined from the real human plasma samples obtained from 22 volunteers, included in this study, after the administration of a single oral dose. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Editura Academiei Romane | en_US |
dc.title | Determination of nimesulide and its active metabolite in plasma samples based on solvent deproteinization and HPLC-DAD analysis | en_US |
dc.type | Article | en_US |
item.grantfulltext | open | - |
item.languageiso639-1 | en_US | - |
item.openairetype | Article | - |
item.fulltext | With Fulltext | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
Appears in Collections: | Articles |
Files in This Item:
File | Description | Size | Format | |
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A ISI RRC NIMES 2007.pdf | 409.65 kB | Adobe PDF | View/Open |
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