The use of a large volume injection of hydrophobic solvents as diluents for less hydrophobic solutes has already been proven for C18 and C8 stationary phases in reversed-phase liquid chromatography. The same possibility is investigated for a phenyl-hexyl stationary phase using aromatic solvents (benzene, toluene, ethylbenzene and propylbenzene) as diluents for several model analytes also containing
aromatic rings. Both hydrophobic interaction and p–p stacking account for the competitive interaction of both the diluent and model analytes with the phenyl-hexyl phase. A linear decrease in analyte retention factor was observed with an increase of injection volume in the range of 1–100 mL. A moderate peak efficiency decrease
was also observed, but peaks of model analytes remained undistorted with minimum band broadening up to 100 mL injection volume. A very small retention decrease was observed when changing the sample diluent in the homologous series: benzene, toluene, ethylbenzene and propylbenzene. The critical conditions
for a successful large volume injection of analytes dissolved in studied hydrophobic solvents are for the analyte to have lower hydrophobicity and for the specified solutes to have proper solubility.