This study presents an introduction in air revitalisation practice by using a microalgae-based biosystem. Althrough the study is developed in the light of space applications, it opens new horisons for implementing such systems for terestrial applications as well, where biotechnologies for addresing climate change and other issues associated with air pollution is in high demand. The experiments have been performed at laboratory-scale by using Arthrospira (spirulina) platensis as microalgae model. Influence of culture and illumination conditions, as well initial gas composition and other factors/techniques such culture filtration on the biosystem performance were investigated and the relevant results are presented and discussed. Based on the actual findings, future research needs are addressed.