Total antioxidant capacity of some fruit seeds extracts

The objective of the study has been to determine total antioxidant capacity (TAC) of some fruit seeds extracts by a chemiluminescence (CL) method, based on a luminol/ Co(II)-EDTA/ H2O2 system. Extracts of Citrus x limon (lemon), a variety of Citrus reticulata (clementine), Vitis vinifera (white grapes), and Citrullus lanatus (watermelon), were prepared by refluxing 96 % ethylic alcohol. The calibration curve was drawn by using the quercitin over a concentration between 10-5 –10-3 moles L-1. RSD was 2.65% (n = 10, cquercetin = 3.5 x 10-5 M). TAC values determined for the analyzed seeds extracts were as follows: 603 for lemon, 594 for grapes, 437 for watermelon and 279 for Clementine, all in quercetin mg equivalent/100 g dw. The precision of the method was verified by applying a standard addition method.